Prague, Czech Republic · Startup Adam is a marketplace for tradespeople, effectively an on-demand platform for home renovation and construction services. Customers can book vetted painters, electricians, or renovation crews, while providers receive steady demand and streamlined scheduling. The company has expanded across Europe, including the UK, Spain, and France. By 2026, Adam is a visible Czech marketplace success in the home services sector.
Startup Growth MarketplaceServicesConsumer
Prague, Czech Republic · Startup Apify turns the web into an API by providing a platform where developers build and monetize “actors” that extract data from websites. It has become a critical part of the AI data supply chain, powering scraping, monitoring, and automation at scale. The company bootstrapped to profitability before raising capital and maintains a strong open developer ecosystem. By 2026, Apify is a global leader in web automation infrastructure.
Startup Growth AutomationDeveloper ToolsData
Prague, Czech Republic · Startup Better Stack is a Prague-founded observability platform built by engineers for engineers, led by Juraj Masar (CEO) and Veronika Kolejak (COO). The company is known for combining speed, lower cost, and strong product design in a unified stack that covers uptime monitoring, log management, incident response, and status pages. Its technical positioning emphasizes standard SQL over proprietary query languages and high-performance analytics on ClickHouse-based infrastructure. Better Stack's January 2024 Series A and extension brought total funding to about $28.6M, with Creandum, KAYA Ventures, and Susa Ventures among key investors plus notable angels from Box, Flexport, Neo4j, Yelp, Codecademy, and UiPath. Publicly shared company metrics reported more than 200,000 developers and 4,000+ customers, and by 2026 Better Stack is widely viewed as one of the strongest Czech global DevTools contenders.
Startup Growth DevToolsObservabilityInfrastructure
Prague, Czech Republic · Startup Choice provides QR payments and digital menus for restaurants, enabling faster ordering and higher table turnover. The platform is growing quickly across CEE and Eastern Europe as restaurants digitize front-of-house operations. It reduces staffing overhead while giving restaurants richer customer data. By 2026, Choice is a fast-scaling Czech restaurant-tech player.
Startup Growth Restaurant TechPaymentsSaaS
Prague, Czech Republic · Startup Cyrkl is a circular-economy marketplace described as “Tinder for industrial waste.” It enables factories to sell scrap materials such as plastics or metals to recyclers instead of landfilling them. The platform improves pricing transparency and accelerates reuse of materials across industrial supply chains. By 2026, Cyrkl is a recognized circular-economy player in CEE with growing cross-border adoption.
Startup Growth Circular EconomyMarketplaceSustainability
Prague, Czech Republic · Startup Data, IoT and AI company building custom and pre-made machine-learning solutions for industrial clients in agriculture, manufacturing, biotech and foodtech, with a team of 80+ across Prague and Brno.
Startup AIIoTData Science
Prague, Czech Republic · Startup Daytrip provides door-to-door private car transfers between cities, pairing long-distance travel with local driver-guides. The model serves tourists who want flexible, comfortable intercity travel without the hassle of trains or buses. The company is profitable and has expanded globally, including the US and Asia. By 2026, Daytrip is a recognized Czech travel-tech export.
Startup Growth TravelMobilityMarketplace
Prague, Czech Republic · Startup Cloud-native no-code/low-code business rules engine (BRMS) for decision automation in pricing, credit scoring, claims, fraud and data validation; processes 100M+ decisions daily for clients in 30+ countries.
Startup Seed SaaSFintechDeveloper Tools
Prague, Czech Republic · Startup Deepnote is a collaborative, browser-based data notebook often described as “Figma for data scientists.” It enables teams to explore data, build analyses, and share results in real time without the friction of local environments. By 2026, it is a default tool for modern data teams and has significant traction in the US market. The company is a Czech-born SaaS success story with global adoption.
Startup Growth Data ScienceAnalyticsSaaS
Ústí nad Labem, Czech Republic · Startup Cybersecurity SaaS delivering Zero Trust Architecture as a service for SMBs: business VPN, ZTNA, secure web gateway, MFA and device posture checks, deployable in minutes and used by 1,000+ customers in 120+ countries.
Startup CybersecuritySaaSEnterprise Software
Prague, Czech Republic · Startup Hedepy is an online therapy platform connecting patients with certified psychotherapists via video. It has expanded across more than ten European markets, offering multilingual access and flexible scheduling. The service addresses mental health capacity shortages by making therapy easier to access and scale. By 2026, Hedepy is a leading Czech health-tech company in digital mental health.
Startup Growth HealthTechMental HealthMarketplace
Prague, Czech Republic · Startup Inventoro provides AI-driven sales forecasting and inventory optimization for SMB retailers. The platform recommends what to order, when to reorder, and how to reduce dead stock, helping merchants improve margins. It integrates with POS and ecommerce systems to automate demand planning without enterprise complexity. By 2026, Inventoro is a fast-growing Czech AI retail-tech company.
Startup Growth AIRetailSaaS
Prague, Czech Republic · Startup Keboola provides an all-in-one data operations platform for connecting, cleaning, and orchestrating data pipelines. It is known for flexibility, modularity, and an enterprise-grade pay-as-you-go model. The company has been profitable for years and raised a late Series A to accelerate global expansion. By 2026, Keboola is a trusted DataOps layer for mid-market and enterprise customers.
Startup Growth DataOpsETLEnterprise Software
Brno, Czech Republic · Startup Kiwi.com is a Brno-founded online travel agency that uses proprietary Virtual Interlining technology to combine flights from airlines that do not formally codeshare, creating unique multi-carrier itineraries unavailable on traditional booking platforms. The platform searches across 750+ carriers including low-cost airlines, trains, and buses to find the cheapest routes. Founded in 2012 by Oliver Dlouhy, the company grew rapidly and became one of the largest travel booking platforms in Europe, processing tens of millions of bookings annually. Kiwi.com also offers a Guarantee product that protects travelers on self-transfer connections.
Startup Late Stage Travel TechE-commerce
Brno, Czech Republic · Startup Kontent.ai is a Brno-based headless CMS platform that decouples content creation from front-end presentation, enabling enterprises to manage and deliver content across websites, mobile apps, kiosks, and IoT devices through a unified API. Originally spun out of Kentico Software by founder Petr Palas, the platform combines structured content modeling with AI-powered features for personalization and workflow automation. Kontent.ai serves mid-market and enterprise customers globally, competing with Contentful and Storyblok in the composable digital experience space.
Startup Growth SaaSContent ManagementAI
Prague, Czech Republic · Startup Liftago is a Prague-based ride-hailing and delivery marketplace founded in 2012 that connects passengers and businesses with licensed professional taxi drivers and courier services. Unlike Uber and Bolt, Liftago works exclusively with regulated taxi operators, positioning itself as a compliant alternative in the Czech market. The platform expanded into last-mile delivery and B2B logistics, helping restaurants and retailers fulfill same-day orders. Liftago has become one of the most widely used mobility apps in Prague, with a strong network of professional drivers and fleet partnerships.
Startup Growth MobilityLogisticsMarketplace
Prague, Czech Republic · Startup Malcom Finance (formerly 4Trans) provides factoring and financing for truck drivers and logistics SMEs. Its underwriting leverages real-time telematics and operational data to assess risk more accurately than traditional banks. This enables faster payouts and more flexible credit for transport operators who are cash-flow constrained. By 2026, Malcom is a fast-growing Czech fintech focused on the logistics economy.
Startup Growth FintechLogisticsLending
Prague, Czech Republic · Startup Manta is a data lineage and metadata-management platform that gives organisations visibility into their data environments by automatically scanning the data stack and mapping data flows, sources, transformations and dependencies. By generating this lineage and an audit trail, it helps businesses trace where data originated and how it evolved, and can discover and anonymise sensitive data to reduce regulatory risk. Founded in Prague in 2016, Manta was acquired by IBM in October 2023 and its data lineage was integrated into IBM's watsonx data and AI governance portfolio.
Startup Series B DataEnterprise SoftwareB2B SaaSAI
Brno, Czech Republic · Startup Mewery is a food-tech and biotech startup cultivating pork meat using microalgae-based processes. The hybrid approach reduces the cost and complexity of lab-grown meat by combining cell cultivation with plant inputs. This positions the company to deliver cultured meat products faster and at lower price points than traditional cellular agriculture methods. By 2026, Mewery is a notable Central European pioneer in sustainable protein.
Startup Early Stage FoodTechBiotechClimate
Prague, Czech Republic · Startup Mews is a cloud-native property management system built to replace legacy hotel software with an API-first platform. It powers hotel operations from booking and pricing to payments and guest communications, enabling chains to modernize at speed. By 2026, Mews is aggressively acquiring smaller travel-tech vendors and expanding global enterprise contracts, positioning itself as the default operating system for modern hospitality. The company is a Czech-born unicorn with a strong international footprint and deep Prague engineering roots.
Startup Late Stage HospitalitySaaSTravel
Prague, Czech Republic · Startup Oddin.gg is a B2B provider of esports odds feeds, risk management, and trading tools. It supplies real-time probabilities for esports titles such as Counter-Strike and League of Legends, enabling betting operators to price markets reliably. The company has become a global leader in esports wagering infrastructure. By 2026, Oddin.gg is one of the Czech Republic’s most internationally recognized gaming-tech exports.
Startup Growth EsportsBettingData
Prague, Czech Republic · Startup AI-powered personal finance app that detects unnecessarily high payments and subscriptions in users' bank accounts and recommends better offers. Operating in Czechia and Slovakia, it has passed 350,000 users.
Startup FintechOpen Banking
Prague, Czech Republic · Startup Productboard is a Prague-founded software company building one of the defining systems of record for modern product management. The platform helps teams collect customer feedback, connect it to feature requests, prioritize product decisions, and communicate roadmaps across engineering, sales, and leadership. That matters because product work often suffers from fragmented information spread across support systems, CRM notes, internal docs, and ad hoc spreadsheets. Productboard turns that sprawl into a more structured workflow for deciding what should be built and why. In the Central and Eastern European ecosystem, it is a particularly strong reference case because it proves that category-leading B2B SaaS products can emerge from Prague and scale globally while retaining a major engineering footprint in the region. It also occupies an important position in the software operating stack of growth companies, since fast-moving startups need better ways to align customer demand with product execution. As a result, Productboard is not only a Czech success story, but also a useful link between early-stage product discipline and later-stage organizational scale.
Startup Late Stage SaaSProductivityEnterprise Software
Prague, Czech Republic · Startup AI-powered real-time recommendation and personalized search engine offered as a SaaS API; uses 100+ proprietary ML models and the ReQL query language, serving media, streaming, e-commerce and marketplaces.
Startup Bootstrapped AISaaSE-commerce
Prague, Czech Republic · Startup Resistant AI focuses on AI-driven fraud detection for financial services, specifically identifying manipulated documents used in KYC and onboarding. Its models detect fake paystubs, doctored IDs, and synthetic documents that bypass traditional checks. As digital fraud grows more sophisticated, Resistant AI has become a critical partner for fintechs and banks. By 2026, it is a leading Czech player in the FinTech security category.
Startup Growth FintechCybersecurityAI
Prague, Czech Republic · Startup Rohlik Group is the Czech Republic's flagship ecommerce and logistics scale-up, often compared to Ocado for its vertically integrated model. The company dominates online grocery across Central Europe and operates the Knuspr brand in Germany and Austria, pairing premium assortment with fast delivery windows. By 2026, Rohlik is heavily automating fulfillment to push down unit economics while expanding warehouse capacity across CEE. Its influence is outsized locally, acting as a talent magnet and a repeat-founder factory for the Prague ecosystem.
Startup Late Stage E-GroceryLogisticsMarketplace
Prague, Czech Republic · Startup Rossum specializes in intelligent document processing, moving beyond basic OCR to cognitive data capture. Its AI reads invoices, shipping manifests, and other structured documents with human-like accuracy, automating workflows for logistics, manufacturing, and finance. The company is scaling rapidly in the US market and positioning itself as the backbone for enterprise document automation. By 2026, Rossum is one of the Czech ecosystem's most credible soonicorns.
Startup Growth AIEnterprise SoftwareAutomation
Brno, Czech Republic · Startup Sewio is a Brno-based company specializing in ultra-wideband (UWB) real-time location systems (RTLS) for indoor tracking of assets, vehicles, and people in industrial environments. Its platform delivers centimeter-level positioning accuracy inside warehouses, factories, and logistics centers where GPS signals cannot reach. Sewio's technology is used for workflow optimization, safety zone enforcement, and forklift fleet management by manufacturers and logistics operators worldwide. The company is a recognized leader in the European RTLS market and was acquired by Siemens subsidiary Enlighted in 2022.
Startup Growth IoTIndustrialRTLS
Brno, Czech Republic · Startup Conversational e-commerce platform combining live chat, chatbots and an AI shopping assistant (Mira) to engage visitors and convert leads; used on 100,000+ websites, with leading live-chat market share in Central Europe.
Startup Growth SaaSE-commerceAI
Prague, Czech Republic · Startup PropTech platform unifying workplace apps and building systems (BMS, HVAC, lighting, access, IoT) into one AI-driven layer for space utilization, booking, digital twins and ESG tracking across real estate portfolios.
Startup Growth PropTechIoTSaaS
Prague, Czech Republic · Startup Tatum is a developer platform that unifies dozens of blockchain protocols into a single API, allowing enterprises to build crypto features without specialized blockchain teams. The platform abstracts wallet creation, transactions, and smart contract interactions into simple endpoints. By 2026, Tatum positions itself as the “Stripe for crypto infrastructure,” serving banks and fintechs that want to launch regulated digital-asset services. Its focus is on reliability and developer speed rather than speculative crypto exposure.
Startup Growth BlockchainInfrastructureDeveloper Tools
Prague, Czech Republic · Startup Twisto is a Prague-based fintech founded in 2013 by Michal Smida, offering buy-now-pay-later and digital payment solutions across Central Europe. The platform allows consumers to defer payments at checkout and manage spending via a mobile app and virtual card, while merchants benefit from higher conversion rates. Twisto expanded into Poland and Romania before being acquired by Zip Co (Australia) in 2022 for approximately $97 million. The company helped pioneer BNPL adoption in CEE and remains a notable Czech fintech exit story.
Startup Growth FintechPaymentsBNPL
Prague, Czech Republic · Startup Woltair is a digital marketplace and installation platform for heat pumps and solar systems. It streamlines the complex process of finding qualified contractors, securing subsidies, and completing installations. By 2026, the company is a notable Czech green-tech player pushing heat pump adoption across Central Europe. Its operational advantage is a software-driven workflow layered on top of local installer networks.
Startup Growth GreenTechEnergyMarketplace